TA319493

antibody from OriGene

Targeting: WRNIP1 bA420G6.2, CFAP93, FAP93, FLJ22526, WHIP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319493

Provider

OriGene

Product name

Rabbit polyclonal anti-WHIP antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal anti-WHIP antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

WRNIP1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.10 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB analysis using Anti-Human WHIP antibody to detect Human WHIP present in a HEK293 whole cell lysate.? ~30ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at RT for 2 h followed by detection using IRDye? 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1:5,000 for 45 min.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB analysis using anti-Human WHIP antibody. ?Testing was performed on antiserum prior to affinity purification.? Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WHIP1.? ~30ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE.? Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 kDa is detected. The blot was incubated with a 1:1000 dilution of the antibody.

Validation comment

WB