NBP1-77987

antibody from Novus Biologicals

Targeting: WRNIP1 bA420G6.2, CFAP93, FAP93, FLJ22526, WHIP

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NBP1-77987

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-77987, RRID:AB_11035326

Product name

Rabbit Polyclonal WHIP Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Reactivity is expected against mouse, rat and monkey WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: WHIP Antibody [NBP1-77987] - Antibody with and without pre-incubation with blocking peptide. Testing was performed on antiserum prior to affinity purification. Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WHIP1. ~30ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 kDa is detected. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(R) 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1:5,000 for 45 min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: WHIP Antibody [NBP1-77987] - HEK293 whole cell lysate. ~30ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(R) 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1:5,000 for 45 min.