11773-1-AP

antibody from Proteintech Group

Targeting: NFIL3 E4BP4, IL3BP1, NF-IL3A, NFIL3A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

11773-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11773-1-AP, RRID:AB_2267287

Product name

NFIL3 antibody

Antibody type

Polyclonal

Description

KD/KO validated NFIL3 antibody (Cat. #11773-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Disruption of the biorhythm in gastric epithelial cell triggers inflammation in Helicobacter pylori-associated gastritis by aberrantly regulating NFIL3 via CagA activated ERK-SP1 pathway.

Teng Y, Lv Y, Chen W, Mao F, Peng L, Huang H, Li H, Shi L, Zou Q, Zhuang Y, Tian W, Guo H
Cell communication and signaling : CCS 2025 Jun 15;23(1):285

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A Novel FNDC1-NAMPT-NAD axis is Implicated in Small and Large-vessel Arterial Disease and Drives Vascular Calcification.

Lee S, Guo Y, Kajuluri LP, Roh K, Singh K, Jiang W, Moore E, Tattersfield H, Ostrom K, Birchenough C, Johnson AL, Krinsky S, Bouchouari H, Mohammedeh M, Lee LY, Wyant GA, Rich SS, Rotter JL, Taylor KD, Malik S, Gerszten R, Cruz D, Tracy RP, Wu H, Zhang H, Lu H, Kavousi M, Shanahan C, Duer M, Kramann R, Nazarian RM, Eagleton MJ, Chen Y, Miller CL, Nigwekar SU, Malhotra R
bioRxiv : the preprint server for biology 2025 Jul 10;

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Regulation of Erythroid Differentiation via the HIF1α-NFIL3-PIM1 Signaling Axis Under Hypoxia.

Yu HC, Cui R, Chen MY, Du XY, Bai QR, Zhang SL, Guo JJ, Tong FC, Wu J
Antioxidants & redox signaling 2025 Jan;42(1-3):36-52

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The NF-κB Signalling Regulates the Abnormal Functions of Neutrophils in Severe Periodontal Disease.

Lu W, Tong Y, Cheng J, Zhu X, Li J, Yang C, Gu H, Wu Y, Lin C
International dental journal 2025 Dec;75(6):103973

---

Integrative analysis reveals NFIL3 as a prognostic marker in glioblastoma associated with anesthesia and circadian rhythm genes.

Yuan Y, Lang F, Huang J, Xia Y, Yuan G
Discover oncology 2025 Dec 12;17(1):105

---

Dysregulation of CRY1 impairs brain thyroid hormone pathway and promotes anxiety-like behavior in male mice.

Lv B, Liu Y, Yang Y, Wu Y, Chen Z, Zhang T, Yu F, Shi Y
Metabolism: clinical and experimental 2025 Aug;169:156292

---

Identification of neutrophil extracellular traps-related genes for the diagnosis of acute myocardial infarction based on bioinformatics and experimental verification.

Yuan HJ, Yu YD, Liu WW, Liu XJ, Han QC, Shi JL, Xue YT, Li Y
Journal of inflammation (London, England) 2025 Aug 27;22(1):35

---

Nfil3 contributes to renal fibrosis by activating fibroblasts through directly promoting the expression of Spp1.

Li H, Li P, Shen Q, Zhu Z, Yang M, Zhang X, Yang M, Shen W, Gong W
Biochimica et biophysica acta. Molecular basis of disease 2025 Apr;1871(4):167741

---

Dietary Fiber-Derived Microbial Butyrate Suppresses ILC2-Dependent Airway Inflammation in COPD.

Jiang M, Wang J, Li Z, Xu D, Jing J, Li F, Ding J, Li Q
Mediators of inflammation 2024;2024:6263447

---

Identification and Validation of Hub Genes Related to Neutrophil Extracellular Traps-Mediated Cell Damage During Myocardial Infarction.

Ke D, Ni J, Yuan Y, Cao M, Chen S, Zhou H
Journal of inflammation research 2024;17:617-637

---

A pan-cancer characterization of immune-related NFIL3 identifies potential predictive biomarker.

Fei Q, Zhang X, Wang S, Shu G, Yin G
Journal of Cancer 2024;15(5):1271-1286

---

Multi-omic human pancreatic islet endoplasmic reticulum and cytokine stress response mapping provides type 2 diabetes genetic insights.

Sokolowski EK, Kursawe R, Selvam V, Bhuiyan RM, Thibodeau A, Zhao C, Spracklen CN, Ucar D, Stitzel ML
Cell metabolism 2024 Nov 5;36(11):2468-2488.e7

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E4BP4 in macrophages induces an anti-inflammatory phenotype that ameliorates the severity of colitis.

Kajimura Y, Taguchi A, Nagao Y, Yamamoto K, Masuda K, Shibata K, Asaoka Y, Furutani-Seiki M, Tanizawa Y, Ohta Y
Communications biology 2024 May 7;7(1):527

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E4bp4-Cyp3a11 axis in high-fat diet-induced obese mice with weight fluctuation.

Sun S, Zhang R, Chen Y, Xu Y, Li X, Liu C, Chen G, Wei X
Nutrition & metabolism 2024 May 27;21(1):30

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Hydrogen sulfide attenuates atherosclerosis induced by low shear stress by sulfhydrylating endothelium NFIL3 to restrain MEST mediated endothelial mesenchymal transformation.

Zhou K, Luo W, Gui DD, Ren Z, Wei DH, Liu LS, Li GH, Tang ZH, Xiong WH, Hu HJ, Jiang ZS
Nitric oxide : biology and chemistry 2024 Jan 1;142:47-57

---

Long non-coding RNA LncCplx2 regulates glucose homeostasis and pancreatic β cell function.

Wang L, Hu L, Wang X, Geng Z, Wan M, Hao J, Liu H, Fan Y, Xu T, Li Z
Molecular metabolism 2024 Feb;80:101878

---

Identification of hypoxia- and immune-related biomarkers in patients with ischemic stroke.

Zhang H, Sun J, Zou P, Huang Y, Yang Q, Zhang Z, Luo P, Jiang X
Heliyon 2024 Feb 29;10(4):e25866

---

Deciphering the diurnal rhythm regulating mechanism of flavin-containing monooxygenase 3 in mouse liver.

Huang M, Duan S, Zhang Q, Guo L, Qin Z, Yang J
The international journal of biochemistry & cell biology 2024 Apr;169:106538

---

Jujuboside A ameliorates cognitive deficiency in delirium through promoting hippocampal E4BP4 in mice.

Du J, Zhang F, Chen M, Xiao Y, Zhang L, Dong L, Dong D, Wu B
The Journal of pharmacy and pharmacology 2023 Jul 5;75(7):886-897

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Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice.

Chen M, Xiao Y, Zhang F, Du J, Zhang L, Li Y, Lu D, Wang Z, Wu B
Biochemical pharmacology 2022 Nov;205:115286

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Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription.

Yang W, Li J, Zhang M, Yu H, Zhuang Y, Zhao L, Ren L, Gong J, Bi H, Zeng L, Xue Y, Yang J, Zhao Y, Wang S, Gao S, Fu Z, Li D, Zhang J, Wang T, Shan M, Tang B, Li X
Journal of experimental & clinical cancer research : CR 2022 Feb 18;41(1):67

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NFIL3 Facilitates Neutrophil Autophagy, Neutrophil Extracellular Trap Formation and Inflammation During Gout via REDD1-Dependent mTOR Inactivation.

Tang H, Tan C, Cao X, Liu Y, Zhao H, Liu Y, Zhao Y
Frontiers in medicine 2021;8:692781

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Identification of rhythmic human CYPs and their circadian regulators using synchronized hepatoma cells.

Chen M, Zhou C, Zhang T, Wu B
Xenobiotica; the fate of foreign compounds in biological systems 2020 Sep;50(9):1052-1063

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The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition.

Yu F, Zhang T, Zhou C, Xu H, Guo L, Chen M, Wu B
Theranostics 2019;9(10):2754-2767

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NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression.

Xu J, Xu G, Zhang T, Chen T, Zhao W, Wang G
BioMed research international 2019;2019:4068521

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The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.

Chen M, Guan B, Xu H, Yu F, Zhang T, Wu B
Drug metabolism and disposition: the biological fate of chemicals 2019 Nov;47(11):1333-1342

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The transcription factor E4bp4 regulates the expression and activity of Cyp3a11 in mice.

Tong Y, Zeng P, Zhang T, Zhao M, Yu P, Wu B
Biochemical pharmacology 2019 May;163:215-224

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Retigabine ameliorates acute stress-induced impairment of spatial memory retrieval through regulating USP2 signaling pathways in hippocampal CA1 area.

Li C, Zhang J, Xu H, Chang M, Lv C, Xue W, Song Z, Zhang L, Zhang X, Tian X
Neuropharmacology 2018 Jun;135:151-162

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Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice.

Shiihashi G, Ito D, Yagi T, Nihei Y, Ebine T, Suzuki N
Brain : a journal of neurology 2016 Sep;139(Pt 9):2380-94

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 11773-1-AP(NFIL3 antibody) at dilution of 1:1000

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The NFIL3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NFIL3. This antibody recognizes human,mouse,rat antigen. The NFIL3 antibody has been validated for the following applications: ELISA, WB, IP analysis.

Appendix

11773-1-AP.pdf

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