Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 698102 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#698102, RRID:AB_2687267
- Product name
- Purified anti-NFIL3
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- W16121C
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total lysates (15µg protein) from Jurkat (negative control), HepG2 and NIH3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:250 (2µg/ml), 1:500 (1µg/ml) and 1:1000 (0.5µg/ml) purified anti-NFIL3 antibody, clone W16121C (upper). Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder.
- Conjugate
- Unconjugated