PA5-17978

antibody from Invitrogen Antibodies

Targeting: NFIL3 E4BP4, IL3BP1, NF-IL3A, NFIL3A

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17978

Provider

Invitrogen Antibodies

Product name

NFIL3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 2,000.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot staining of HepG2 cell lysate using Product # PA5-17978 at a concentration of 0.5 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFIL3 by a NFIL3 monoclonal antibody (Product # PA5-17978) at a concentration of 1 µg/mL. Jurkat nuclear cell lysate (RIPA buffer, 20µg total protein per lane). Detected using chemiluminescence.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of NFIL3 in Human peripheral blood mononuclear cells using a polyclonal antibody (Product #PA5-17978). Human peripheral blood mononuclear cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.