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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00007702-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007702-M01, RRID:AB_425746
- Product name
- ZNF143 monoclonal antibody (M01), clone 2B4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant ZNF143.
- Antigen sequence
MTEFPGGGMEAQHVTLCLTEAVTVADGDNLENMEG
VSLQAVTLADGSTAYIQHNSKDAKLIDGQVIQLED
GSAAYVQHVPIPKSTGDSLRLEDGQAVQLEDGTTA
FIHHTSKDSYDQSALQAVQLEDGTTAYIHHAVQVP
QSDTILAIQADGTVAGLHTGDATIDPDTISALEQY
AAKVSIDGSESVAGTGMIGENEQEKKMQIVLQGHA
TRVTAKSQQSGEKAFRCEYDGCGKLYTTAHHLKVH
ERSHTGDRPYQCEHAGCGKAFATGYGLKSHVRTHT
GEKPYRCSEDNCTKSFKTSGDLQKHIRTHTGERPF
KCPFEGCGRSFTTSNIRKVHVRTHTGERPYYCTEP
GCGRAFASATNYKNHVRIHTGEKPYVCTVPGCDKR
FTEYSSLYKHHVVHTHSKPYNCNHCGKTYKQISTL
VMHKRTAHNDTEPIEEEQEAFFEPPPGQGEDVLKG
SQITYVTGVEGDDVVSTQVATVTQSGLSQQVTLIS
QDGTQHVNISQADMQAIGNTITMVTQDGTPITVPA
HDAVISSAGTHSVAMVTAEGTEGQQVAIVAQDLAA
FHTASSEMGHQQHSHHLVTTETRPLTLVATSNGTQ
IAVQLGEQPSLEEAIRIASRIQQGETPGLDD- Isotype
- IgG
- Antibody clone number
- 2B4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin.
Zfp143 regulates Nanog through modulation of Oct4 binding.
Vinckevicius A, Parker JB, Chakravarti D
Molecular and cellular biology 2015 Dec;35(24):4135-46
Molecular and cellular biology 2015 Dec;35(24):4135-46
Zfp143 regulates Nanog through modulation of Oct4 binding.
Chen X, Fang F, Liou YC, Ng HH
Stem cells (Dayton, Ohio) 2008 Nov;26(11):2759-67
Stem cells (Dayton, Ohio) 2008 Nov;26(11):2759-67
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ZNF143 monoclonal antibody (M01), clone 2B4 Western Blot analysis of ZNF143 expression in Hela S3 NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ZNF143 is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ZNF143 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to ZNF143 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
