TA800077

antibody from OriGene

Targeting: SERPINB1 anti-elastase, EI, ELANH2, PI2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA800077

Provider

OriGene

Proper citation

OriGene Cat#TA800077, RRID:AB_2625202

Product name

SERPINB1 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)

Antibody type

Monoclonal

Description

SERPINB1 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

SERPINB1

Isotype

IgG

Antibody clone number

OTI1D1

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB1 (RC202138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-SERPINB1 mouse monoclonal antibody (TA800077) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SERPINB1(RC202138).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-SERPINB1 antibody(TA800077),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC202138 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SERPINB1 antibody(TA800077), and then analyzed by flow cytometry.

Validation comment

FC