H00000613-D01P

antibody from Abnova Corporation

Targeting: BCR ALL, BCR1, CML, D22S11, D22S662, PHL

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000613-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000613-D01P, RRID:AB_1673047

Product name

BCR purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human BCR protein.

Antigen sequence

MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQEL
ERCKASIRRLEQEVNQERFRMIYLQTLLAKEKKSY
DRQRWGFRRAAQAPDGASEPRASASRPQPAPADGA
DPPPAEEPEARPDGEGSPGKARPGTARRPGAAASG
ERDDRGPPASVAALRSNFERIRKGHGQPGADAEKP
FYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQM
ERKKSQHGAGSSVGDASRPPYRGRSSESSCGVDGD
YEDAELNPRFLKDNLIDANGGSRPPWPPLEYQPYQ
SIYVGGMMEGEGKGPLLRSQSTSEQEKRLTWPRRS
YSPRSFEDCGGGYTPDCSSNENLTSSEEDFSSGQS
SRVSPSPTTYRMFRDKSRSPSQNSQQSFDSSSPPT
PQCHKRHRHCPVVVSEATIVGVRKTGQIWPNDGEG
AFHGDADGSFGTPPGYGCAADRAEEQRRHQDGLPY
IDDSPSSSPHLSSKGRGSRDALVSGALESTKASEL
DLEKGLEMRKWVLSGILASEETYLSHLEALLLPMK
PLKAAATTSQPVLTSQQIETIFFKVPELYEIHKEF
YDGLFPRVQQWSHQQRVGDLFQKLASQLGVYRAFV
DNYGVAMEMAEKCCQANAQFAEISENLRARSNKDA
KDPTTKNSLETLLYKPVDRVTRSTLVLHDLLKHTP
ASHPDHPLLQDALRISQNFLSSINEEITPRRQSMT
VKKGEHRQLLKDSFMVELVEGARKLRHVFLFTDLL
LCTKLKKQSGGKTQQYDCKWYIPLTDLSFQMVDEL
EAVPNIPLVPDEELDALKIKISQIKSDIQREKRAN
KGSKATERLKKKLSEQESLLLLMSPSMAFRVHSRN
GKSYTFLISSDYERAEWRENIREQQKKCFRSFSLT
SVELQMLTNSCVKLQTVHSIPLTINKEDDESPGLY
GFLNVIVHSATGFKQSSNLYCTLEVDSFGYFVNKA
KTRVYRDTAEPNWNEEFEIELEGSQTLRILCYEKC
YNKTKIPKEDGESTDRLMGKGQVQLDPQALQDRDW
QRTVIAMNGIEVKLSVKFNSREFSLKRMPSRKQTG
VFGVKIAVVTKRERSKVPYIVRQCVE


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BCR expression in transfected 293T cell line (H00000613-T02) by BCR MaxPab polyclonal antibody.Lane 1: BCR transfected lysate(121.00 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between BCR and PIK3CG. HeLa cells were stained with anti-BCR rabbit purified polyclonal 1:1200 and anti-PIK3CG mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)