Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF5708 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Hairless Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Hairless in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Development of versatile non-homologous end joining-based knock-in module for genome editing.
Sawatsubashi S, Joko Y, Fukumoto S, Matsumoto T, Sugano SS
Scientific reports 2018 Jan 12;8(1):593
Scientific reports 2018 Jan 12;8(1):593
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Hairless by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Hairless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5708) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Hairless at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.