PA5-33438

antibody from Invitrogen Antibodies

Targeting: CMKLR1 RVER1

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-33438 - Provider product page
Provider: Invitrogen Antibodies
Product name: CMKLR1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Percent identity with other species by BLAST analysis: Human, Gibbon (100%) Marmoset, Rabbit (95%) Horse (84%).
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 50 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CMKLR1 protein structure - PA5-33438 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 SPM receptors are differentially expressed on the endothelium and the infiltrating leukocytes -- ALX/FPR2 and ChemR23. Acute inflammation was triggered in the ventral aspect of forearm of healthy volunteers by the intradermal injection of 1.5 x 10 7 UV-killed E . coli (UVkEc) suspended in 100 mul of sterile saline. Four hours after injection, a 3-mm skin punch biopsy was taken from the inflamed site under local anesthesia. Naive skin was treated as baseline. Formalin-fixed paraffin-embedded skin sections were probed by immunohistochemistry for receptor identification. Low-magnification (x5) and high-magnification (x40) images at baseline and at the 4-hour time point are shown here for ALX/FPR2 ( A ) and ChemR23 ( B ). Red arrows highlight the endothelium and black arrow highlights the infiltrating leukocytes. Representative images from n = 3. SPM, specialized pro-resolving mediator.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 SPM receptor expression increases during acute inflammation. Acute inflammation was triggered in the ventral aspect of forearms of healthy volunteers by the intradermal injection of 1.5 x 10 7 UV-killed E . coli (UVkEc) suspended in 100 mul of sterile saline. Four hours after injection, a 3-mm skin punch biopsy was taken from the inflamed site under local anaesthesia. Formalin-fixed paraffin-embedded skin sections were probed by immunohistochemistry for receptor identification, as shown in Figures 4 and 5 . Increase in receptor expression from baseline to 4 hours following UVkEc injection for ALX/FPR2 ( A ), ChemR23 ( B ), GPR18 ( C ), and GPR32 ( D ) is shown here. Data expressed as box-and-whisker plots (box representing the median and the whiskers representing the maximum-minimum values). Statistical comparison between baseline and 4-hour expression was assessed by Wilcoxon's matched-pairs test. n = 3 for each time point. * P < 0.05. SPM, specialized pro-resolving mediator.