Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF6277 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal HNF-6/ONECUT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human HNF-6/ONECUT1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human ONECUT2 is observed.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Onecut-dependent Nkx6.2 transcription factor expression is required for proper formation and activity of spinal locomotor circuits.
The Onecut Transcription Factors Regulate Differentiation and Distribution of Dorsal Interneurons during Spinal Cord Development.
Toch M, Harris A, Schakman O, Kondratskaya E, Boulland JL, Dauguet N, Debrulle S, Baudouin C, Hidalgo-Figueroa M, Mu X, Gow A, Glover JC, Tissir F, Clotman F
Scientific reports 2020 Jan 22;10(1):996
Scientific reports 2020 Jan 22;10(1):996
The Onecut Transcription Factors Regulate Differentiation and Distribution of Dorsal Interneurons during Spinal Cord Development.
Kabayiza KU, Masgutova G, Harris A, Rucchin V, Jacob B, Clotman F
Frontiers in molecular neuroscience 2017;10:157
Frontiers in molecular neuroscience 2017;10:157
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human HNF-6/ONECUT1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HNF-6/ONECUT1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.