PA5-96622

antibody from Invitrogen Antibodies

Targeting: BCAS3 FLJ20128

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96622

Provider

Invitrogen Antibodies

Product name

BCAS3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LAGLVPPGSP GPITRHGSYD SLASDHSGQE DEEWLSQVEI VTHTGPHRRL WMGPQFQFKT IHPSGQTTVI SSSSSVLQSH GPSDTPQPLL DFDTDDLDLN SLRIQPVRSD PVSMPGSSRP VSDRRGVSTV IDAASGTFDR SVTLLEVCGS WPEGFGLRHM SSMEHTEEGL RERLADAMAE SPSRDVVGSG TELQREGSIE TLSNSSGSTS GSIPRNFDGY RSPLPTNESQ PLSLFPTGFP; Positive Samples: HeLa, BT474, Rat brain; Cellular Location: Cytoplasm, Nucleus, cytoskeleton

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.54 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of BCAS3 in A-549 cells. Samples were incubated with BCAS3 Polyclonal antibody (Product # PA5-96622). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of BCAS3 in 200 μg extracts of HeLa cells. Samples were precipitated with 3 μg BCAS3 Polyclonal antibody (Product # PA5-96622). Western blot was performed from the immunoprecipitate using BCAS3 Polyclonal antibody (Product # PA5-96622) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of BCAS3 in paraffin-embedded human kidney cancer. Samples were incubated with BCAS3 Polyclonal antibody (Product # PA5-96622) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of BCAS3 in paraffin-embedded human stomach. Samples were incubated with BCAS3 Polyclonal antibody (Product # PA5-96622) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of BCAS3 in paraffin-embedded mouse kidney. Samples were incubated with BCAS3 Polyclonal antibody (Product # PA5-96622) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of BCAS3 in paraffin-embedded human stomach. Samples were incubated with BCAS3 Polyclonal antibody (Product # PA5-96622) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of BCAS3 in paraffin-embedded mouse kidney. Samples were incubated with BCAS3 Polyclonal antibody (Product # PA5-96622) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of BCAS3 in paraffin-embedded human kidney cancer. Samples were incubated with BCAS3 Polyclonal antibody (Product # PA5-96622) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of BCAS3 was performed in 200 µg extracts of HeLa cells using BCAS3 Polyclonal Antibody (Product # PA5-96622). Western blot was performed from the immunoprecipitate using BCAS3 Polyclonal Antibody.