711097

antibody from Invitrogen Antibodies

Targeting: NR1H2 LXR-b, LXRb, NER, NER-I, RIP15, UNR

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711097

Provider

Invitrogen Antibodies

Product name

LXR beta Recombinant Polyclonal Antibody (8 HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Cat and Bat

Antibody clone number

8 HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on nuclear extracts (30 µg lysate) of Jurkat (Lane 1), PC-3 (Lane 2), A549 (Lane 3), HeLa (Lane 4), HEK-293 (Lane 5), U-87 MG (Lane 6) and tissue extract of mouse muscle (Lane 7). The blots were probed with Anti-NR1h2/ LXRb Recombinant Rabbit Polyclonal Antibody (Product # 711097, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 51 kDa band corresponding to NR1h2/ LXRb was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot®2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).