RQ6202

antibody from NSJ Bioreagents

Targeting: NR1H2 LXR-b, LXRb, NER, NER-I, RIP15, UNR

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6202

Provider

NSJ Bioreagents

Product name

LXR beta Antibody / NR1H2

Antibody type

Polyclonal

Description

This highly specific LXR beta antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence/Direct ELISA applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the LXR beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human PC-3, 2) human HL60, 3) mouse liver and 4) mouse RAW264.7 lysate with LXR beta antibody. Predicted molecular weight ~51 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human MCF7 cells with LXR beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-2 OS cells with LXR beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LXR beta antibody.