AF7868

antibody from R&D Systems

Targeting: NQO2 DHQV, DIA6, NMOR2, QR2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7868

Provider

R&D Systems

Product name

Human NQO-2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human NQO-2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human NQO-1 is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

P16083


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human NQO-2 by Western Blot. Western blot shows lysates of human liver tissue, human kidney tissue, HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human NQO-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7868) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NQO-2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.