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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-27783 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRPF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, BRPF1 tansfected 293T cell. Predicted reactivity: Mouse (97%), Rat (96%), Chicken (89%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.19 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes.
Truncated BRPF1 Cooperates with Smoothened to Promote Adult Shh Medulloblastoma.
Histone Acetyltransferase KAT6A Upregulates PI3K/AKT Signaling through TRIM24 Binding.
Molecular mechanisms of transcriptional regulation by the nuclear zinc-finger protein Zfat in T cells.
Ishikura S, Nagai M, Tsunoda T, Nishi K, Tanaka Y, Koyanagi M, Shirasawa S
Journal of cellular biochemistry 2021 Jun;122(6):626-638
Journal of cellular biochemistry 2021 Jun;122(6):626-638
Truncated BRPF1 Cooperates with Smoothened to Promote Adult Shh Medulloblastoma.
Aiello G, Ballabio C, Ruggeri R, Fagnocchi L, Anderle M, Morassut I, Caron D, Garilli F, Gianno F, Giangaspero F, Piazza S, Romanel A, Zippo A, Tiberi L
Cell reports 2019 Dec 17;29(12):4036-4052.e10
Cell reports 2019 Dec 17;29(12):4036-4052.e10
Histone Acetyltransferase KAT6A Upregulates PI3K/AKT Signaling through TRIM24 Binding.
Lv D, Jia F, Hou Y, Sang Y, Alvarez AA, Zhang W, Gao WQ, Hu B, Cheng SY, Ge J, Li Y, Feng H
Cancer research 2017 Nov 15;77(22):6190-6201
Cancer research 2017 Nov 15;77(22):6190-6201
Molecular mechanisms of transcriptional regulation by the nuclear zinc-finger protein Zfat in T cells.
Ishikura S, Tsunoda T, Nakabayashi K, Doi K, Koyanagi M, Hayashi K, Kawai T, Tanaka Y, Iwaihara Y, Luo H, Nishi K, Okamura T, Shirasawa S
Biochimica et biophysica acta 2016 Nov;1859(11):1398-1410
Biochimica et biophysica acta 2016 Nov;1859(11):1398-1410
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BRPF1 Polyclonal Antibody (Product # PA5-27783). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRPF1 Polyclonal Antibody (Product # PA5-27783) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of BRPF1 expression in transfected 293T cell line by BRPF1 polyclonal antibody. Lane A: Non-transfected lysate. Lane B: BRPF1 transfected lysate. 7.5% SDS PAGE. BRPF1 Polyclonal Antibody (Product # PA5-27783) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRPF1 Polyclonal Antibody detects Peregrin protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Peregrin stained by BRPF1 Polyclonal Antibody (Product # PA5-27783) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
