MA1-4670

antibody from Invitrogen Antibodies

Targeting: RARB HAP, NR1B2, RRB2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-4670

Provider

Invitrogen Antibodies

Product name

RARB Monoclonal Antibody (336)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with canine, guinea pig, mouse and non-human primate based on 100% sequence homology. This antibody is specific for the ~48 kDa RXR-b protein.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

336

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Differential expression of retinoic acid receptor beta (RARbeta) and the AP-1 transcription factor in normal, premalignant and malignant human laryngeal tissues.

Karamouzis MV, Sotiropoulou-Bonikou G, Vandoros G, Varakis I, Papavassiliou AG
European journal of cancer (Oxford, England : 1990) 2004 Mar;40(5):761-73

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot detection of brain lysate showing specific immunolabeling of the ~48 kDa RXR-3 protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of RARB in rat hippocampal lysate showing specific immunolabeling of a ~48 kDa band corresponding to RARB monoclonal antibody (Product # MA1-4670).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-RARB Monoclonal Antibody (336) (Product # MA1-4670) and 58, 37 kDa bands corresponding to RARB was observed across the cell lines tested and induced upon Retinoic acid treatment in Dendritic cells differentiated from THP-1. Whole cell lysates (30ug lysate) of THP-1 (Lane 1), THP-1 cells differentiated to Dendritic cells (Lane2), Dendritic cells treated with Retinoic acid (1uM for 24 hr) (Lane 3), A549 (Lane 4), SH-SY5Y (Lane 5), MCF7 (Lane 6) and IMR-32 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).