702901

antibody from Invitrogen Antibodies

Targeting: IGLL1 14.1, CD179B, IGL5, IGLL, IGVPB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

702901

Provider

Invitrogen Antibodies

Product name

CD179b Recombinant Rabbit Monoclonal Antibody (8H16L35)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Cat, Pig, Rat.

Antibody clone number

8H16L35

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of JM1 (Lane 1), Daudi (Lane 2), EB2 (Lane 3) and IM-9 (Lane 4). The blot was probed with Anti-CD179b Recombinant Rabbit Monoclonal Antibody (Product # 702901, 1:10000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 23 kDa corresponding to CD179b was observed in relevant cell lines tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, JM1 cells were fixed and permeabilized for detection of endogenous CD179b using Anti-CD179b Recombinant Rabbit Monoclonal Antibody (Product # 702901,1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Panel a-d) clearly demonstrates membrane localization of CD179b in JM1 cells. Panel e-h) shows no specific staining in Raji cells which is CD179b negative, demonstrating specificity. The images were captured at 60X magnification.