25315-1-AP

antibody from Proteintech Group

Targeting: RUNX1 AML1, AMLCR1, CBFA2, PEBP2A2

Provider product page for 25315-1-AP

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Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [49]
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Product number: 25315-1-AP - Provider product page
Provider: Proteintech Group
Product name: RUNX1 (middle) antibody
Antibody type: Polyclonal
Description: RUNX1 (middle) antibody (Cat. #25315-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

RUNX1 protein structure - 25315-1-AP shown in red.

Submitted references Exercise Attenuates Aortic Dissection Via PDE5A-Mediated Inhibition of Vascular Smooth Muscle Cell Phenotypic Switch.

Zhang Y, Wu C, Du D, Hu J, Li Z, Wang Y, Li B, Mu J, Chen X, Yu Y, Wu N, Zuo S
Arteriosclerosis, thrombosis, and vascular biology 2026 May;46(5):e324293

RUNX1 restrains STAT1-GITRL signaling to shape an immunosuppressive CRC microenvironment.

He W, Zheng L, Huang W, Li K, Chen Y, Zeng R, Lin Z, Xu Y, Hu S, Zhang Q
Cell death discovery 2026 Mar 25;12(1)

MSMP promotes an aberrant phenotype of fibroblast-like synoviocytes in rheumatoid arthritis by blocking autophagy via the RUNX1/GPR137B axis.

Wang C, Zeng Y, Tan P, Yuan S, Li W, Ye J, Chen M, Lao M, Xiao Y, Liang L, Xu H, Cai X
Arthritis research & therapy 2026 Feb 3;28(1)

Farrerol inhibits ferroptosis and protects against LPS-induced acute lung injury by targeting the RUNX1/SLC7A11 axis.

Yang Y, Deng S, Liu T, Yin Q, Yang M, Zhang X, Jiang Z, Wang X, Zhang S, Zhang T, Wu D, Xu Y
Frontiers in immunology 2025;16:1720843

Ferulic acid promotes thrombopoiesis via TLR4/JAK2/STAT3 signaling: A novel therapeutic strategy for thrombocytopenia.

Liao R, Huang M, Liu Z, Wang X, Gan X, Hao L, Jiang P, Luo J, Huang Q, Mei Q, Wu A, Wang L, Wu J
Biochemical pharmacology 2025 Sep;239:117088

Chronic ethanol exposure induces hippocampal neuroinflammation and neuronal damage via the astrocytic RUNX1/TOLLIP/TLR3 pathway.

Shen H, Zhang Q, Lv Q, Liu H, Wang C, Meng F, Guo Y, Pei J, Yu C, Tie J, Chen X, Zhang C, Yu H, Wang X
Brain, behavior, and immunity 2025 Nov;130:106081

Modulation of Osteogenic Differentiation by CYBB in Osteoporotic Models.

Wang Z, Xu C, Liu Y, Duan K, Zhu Z, Guan J
IUBMB life 2025 May;77(5):e70023

RUNX1 promotes colorectal cancer progression by activating SERPINE1 transcription.

Yang Y, Deng S, Yin Q, Zhang T, Liu T, Wu D, Xu Y
Biochemical and biophysical research communications 2025 May 26;760:151732

Genome-wide analysis reveals porcine LIFR regulated by DNA methylation promotes the implantation process via the STAT3 signaling.

Zhou C, Huang S, Zheng S, Pius L, Liu M, Xu D
International journal of biological macromolecules 2025 Mar;295:139450

Circ_0044362 Facilitates the Progression of Epithelial Ovarian Cancer via Enhancing HOXB4 Transcription to Activate the RUNX1/IGFBP3 Axis.

Ye S, Wu H, Liu J, Zhou J, He S, Li N
Molecular carcinogenesis 2025 Jun;64(6):1013-1024

PRMT1 inhibition enhances the cardioprotective effect of adipose-derived mesenchymal stem cells against myocardial infarction through RUNX1.

Du D, Wang T, Sun Y, Zhang Y, Zhang B, Shao J, Yang M, Huang C, Zuo S, Wu N
Stem cell research & therapy 2025 Jun 3;16(1):279

DDX5 super-enhancer promotes vasculogenic mimicry formation and metastasis in nasopharyngeal carcinoma by enhancing ADAM10 transcription.

Xia T, Yin H, Zhu Q, Zhang K, Xie H, Shan Y, Zhang S, Zhu R, Li K, Miao M, Lu Y, Wang Z, Zhao J, You Y, You B
Cell reports. Medicine 2025 Jun 17;6(6):102146

Oxymatrine, a novel TLR2 agonist, promotes megakaryopoiesis and thrombopoiesis through the STING/NF-κB pathway.

Ni C, Zhou L, Yang S, Ran M, Luo J, Cheng K, Huang F, Tang X, Xie X, Qin D, Mei Q, Wang L, Xiao J, Wu J
Journal of pharmaceutical analysis 2025 Jan;15(1):101054

RUNX1 promotes proliferation of cervical cancer through TGFB2-MAPK pathway.

Jia Y, Yang N, Tang S, Deng L, Wang Y, Cai X
Scientific reports 2025 Jan 2;15(1):497

Microglial RUNX1/RBM47 ablation inhibits neuronal ferroptosis via regulating the cGAS-STING-MEF2C pathway in mice with postoperative cognitive dysfunction.

Wang S, Wu Z, Song W, Zhang H, Bu X, Gao W, Wu K, Xia Z, Zhao B, Wang W
Communications biology 2025 Dec 17;8(1):1813

IL-23 Receptor Agonism by Mulberroside C Activates the RASGRP1/RAS/ERK Pathway Contributing to Leukopenia Treatments.

Zhang LW, Liu YZ, Li TC, Li JY, Yan XY, Liu ZX, Li LY, Shen HP, Zou WJ, Wu JM
Phytotherapy research : PTR 2025 Aug;39(8):3578-3600

RUNX1 Is an Independent Prognostic Marker Involved in Immune Infiltration and Therapeutic Responses in Clear Cell Renal Cell Carcinoma Patients Through Cluster Regulation With RUNX2.

Jian J, Zhang J, Xu H, Wang H, Hou J
Immunity, inflammation and disease 2025 Aug;13(8):e70242

RUNX1/SLAMF3 Axis Drives Immunosuppression to Contribute to Colorectal Cancer Liver Metastasis by Blocking Phagocytosis and Depleting C1QC(+) Tumor-Associated Macrophages.

Luo Y, Jin X, Huang L, Zeng D, Zhang N, Tang S, Luo S, Syed SE, Dai R, Li Q, Liang S
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Aug;12(32):e06641

Genistin: A Novel Estrogen Analogue Targeting ERβ to Alleviate Thrombocytopenia.

Tang X, Liao R, Zhou L, Yi T, Ran M, Luo J, Huang F, Wu A, Mei Q, Wang L, Huang X, Wu J
International journal of biological sciences 2024;20(6):2236-2260

RUNX1-PDIA5 Axis Promotes Malignant Progression of Glioblastoma by Regulating CCAR1 Protein Expression.

Ji Q, Tu Z, Liu J, Zhou Z, Li F, Zhu X, Huang K
International journal of biological sciences 2024;20(11):4364-4381

Brg1 and RUNX1 synergy in regulating TRPM4 channel in mouse cardiomyocytes.

Ban T, Dong X, Ma Z, Jin J, Li J, Cui Y, Fu Y, Wang Y, Xue Y, Tong T, Zhang K, Han Y, Shen M, Zhao Y, Zhao L, Xiong L, Lv H, Liu Y, Huo R
Frontiers in pharmacology 2024;15:1494205

Calcipotriol abrogates TGF-β1/pSmad3-mediated collagen 1 synthesis in pancreatic stellate cells by downregulating RUNX1.

Zheng M, Li H, Sun L, Cui S, Zhang W, Gao Y, Gao R
Toxicology and applied pharmacology 2024 Oct;491:117078

Harmine promotes megakaryocyte differentiation and thrombopoiesis by activating the Rac1/Cdc42/JNK pathway through a potential target of 5-HTR2A.

Liu X, Lai J, Zhang X, Wu A, Zhou L, Li Y, Huang Q, Huang X, Li H, Lan C, Liu J, Huang F, Wu J
Phytotherapy research : PTR 2024 Nov;38(11):5134-5149

Alnustone promotes megakaryocyte differentiation and platelet production via the interleukin-17A/interleukin-17A receptor/Src/RAC1/MEK/ERK signaling pathway.

Li Y, Lai J, Ran M, Yi T, Zhou L, Luo J, Liu X, Tang X, Huang M, Xie X, Li H, Yang Y, Zou W, Wu J
European journal of pharmacology 2024 May 15;971:176548

RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31.

Tian W, Zhao J, Zhang X, Li P, Li X, Hong Y, Li S
Scientific reports 2024 Jun 17;14(1):13906

Decreasing circ_0014614 promotes the differentiation of bone marrow flineage cells into megakaryocytes in essential thrombocythemia via activiation of miR-138-5p/caspase3 axis.

Yu G, Chen X, Lu W, Li Y, Chen Y, Yin C, Zheng Z, Huang X, Xu D
Blood cells, molecules & diseases 2024 Jul;107:102855

RUNX1 promotes angiogenesis in colorectal cancer by regulating the crosstalk between tumor cells and tumor associated macrophages.

Guo X, Zhang H, He C, Qin K, Lai Q, Fang Y, Chen Q, Li W, Wang Y, Wang X, Li A, Liu S, Li Q
Biomarker research 2024 Feb 28;12(1):29

6-Gingerol ameliorates alveolar hypercoagulation and fibrinolytic inhibition in LPS-provoked ARDS via RUNX1/NF-κB signaling pathway.

Li Q, Xiao C, Gu J, Chen X, Yuan J, Li S, Li W, Gao D, Li L, Liu Y, Shen F
International immunopharmacology 2024 Feb 15;128:111459

ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss.

Wang J, Fendler NL, Shukla A, Wu SY, Challa A, Lee J, Joachimiak LA, Minna JD, Chiang CM, Vos SM, D'Orso I
Molecular cell 2024 Dec 5;84(23):4538-4557.e12

High expression of RUNX1 in colorectal cancer subtype accelerates malignancy by inhibiting HMGCR.

Chang Z, Liu B, He H, Li X, Shi H
Pharmacological research 2024 Aug;206:107293

Activating SRC/MAPK signaling via 5-HT1A receptor contributes to the effect of vilazodone on improving thrombocytopenia.

Zhou L, Ni C, Liao R, Tang X, Yi T, Ran M, Huang M, Liao R, Zhou X, Qin D, Wang L, Huang F, Xie X, Wan Y, Luo J, Wang Y, Wu J
eLife 2024 Apr 4;13

RUNX1-BMP2 promotes vasculogenic mimicry in laryngeal squamous cell carcinoma via activation of the PI3K-AKT signaling pathway.

Zhu Q, Zhang X, Lu F, Miao S, Zhang C, Liu Z, Gao Z, Qi M, An X, Geng P, Wang S, Ren H, Han F, Zhang R, Zha D
Cell communication and signaling : CCS 2024 Apr 12;22(1):227

Intergenic CircRNA Circ_0007379 Inhibits Colorectal Cancer Progression by Modulating miR-320a Biogenesis in a KSRP-Dependent Manner.

Long F, Li L, Xie C, Ma M, Wu Z, Lu Z, Liu B, Yang M, Zhang F, Ning Z, Zhong C, Yu B, Liu S, Wan L, Tian B, Yang K, Guo Y, Chen M, Chou J, Li X, Hu G, Lin C, Zhang Y
International journal of biological sciences 2023;19(12):3781-3803

Vitamin D(3) analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells.

Zheng M, Li H, Gao Y, Brigstock DR, Gao R
European journal of pharmacology 2023 Oct 15;957:176000

RUNX1 promotes proliferation and migration in non-small cell lung cancer cell lines via the mTOR pathway.

Ma H, Jiang S, Yuan Y, Li J, Li Y, Lv Y, Du T, Guan J, Jiang X, Tian L, Zheng Q, Yang L, Li Q
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Nov;37(11):e23195

Targeting UBE2T Potentiates Gemcitabine Efficacy in Pancreatic Cancer by Regulating Pyrimidine Metabolism and Replication Stress.

Jiang X, Ma Y, Wang T, Zhou H, Wang K, Shi W, Qin L, Guan J, Li L, Long B, Wang J, Guan X, Ye H, Yang J, Yu Z, Jiao Z
Gastroenterology 2023 Jun;164(7):1232-1247

miR-9 targeting RUNX1 improves LPS-induced alveolar hypercoagulation and fibrinolysis inhibition through NF-κB inactivation in ARDS.

Xiao C, Li Q, Xiao J, Chen X, Yuan J, Li S, Li W, Gao D, Li L, Liu Y, Shen F
International immunopharmacology 2023 Jul;120:110318

Tumor cell-released kynurenine biases MEP differentiation into megakaryocytes in individuals with cancer by activating AhR-RUNX1.

Zhou L, Wu D, Zhou Y, Wang D, Fu H, Huang Q, Qin G, Chen J, Lv J, Lai S, Zhang H, Tang K, Ma J, Fiskesund R, Zhang Y, Zhang X, Huang B
Nature immunology 2023 Dec;24(12):2042-2052

TRAF5 regulates intestinal mucosal Th1/Th17 cell immune responses via Runx1 in colitis mice.

Li M, Gan C, Zhang R, Wang J, Wang Y, Zhu W, Liu L, Shang J, Zhao Q
Immunology 2023 Dec;170(4):495-509

RUNX1 methylation as a cancer biomarker in differentiating papillary thyroid cancer from benign thyroid nodules.

Li J, Yin Y, Huang H, Li M, Li H, Zhang M, Jiang C, Yang R
Epigenomics 2023 Dec;15(23):1257-1272

Targeting TLR2/Rac1/cdc42/JNK Pathway to Reveal That Ruxolitinib Promotes Thrombocytopoiesis.

Yang S, Tang X, Wang L, Ni C, Wu Y, Zhou L, Zeng Y, Zhao C, Wu A, Wang Q, Xu X, Wang Y, Chen R, Zhang X, Zou L, Huang X, Wu J
International journal of molecular sciences 2022 Dec 17;23(24)

Inhibition of RUNX1 blocks the differentiation of lung fibroblasts to myofibroblasts.

Dubey S, Dubey PK, Umeshappa CS, Ghebre YT, Krishnamurthy P
Journal of cellular physiology 2022 Apr;237(4):2169-2182

Angiotensin II-Induced Long Non-Coding RNA Alivec Regulates Chondrogenesis in Vascular Smooth Muscle Cells.

Samara VA, Das S, Reddy MA, Tanwar VS, Stapleton K, Leung A, Abdollahi M, Ganguly R, Lanting L, Natarajan R
Cells 2021 Oct 9;10(10)

ASCL1 represses a SOX9(+) neural crest stem-like state in small cell lung cancer.

Olsen RR, Ireland AS, Kastner DW, Groves SM, Spainhower KB, Pozo K, Kelenis DP, Whitney CP, Guthrie MR, Wait SJ, Soltero D, Witt BL, Quaranta V, Johnson JE, Oliver TG
Genes & development 2021 Jun;35(11-12):847-869

Pharmacologic targeting of the P-TEFb complex as a therapeutic strategy for chronic myeloid leukemia.

Qing Y, Wang X, Wang H, Hu P, Li H, Yu X, Zhu M, Wang Z, Zhu Y, Xu J, Guo Q, Hui H
Cell communication and signaling : CCS 2021 Aug 9;19(1):83

Identification of lysine acetylome in cervical cancer by label-free quantitative proteomics.

Zhang L, Wang W, Zhang S, Wang Y, Guo W, Liu Y, Wang Y, Zhang Y
Cancer cell international 2020;20:182

CircMUC16 promotes autophagy of epithelial ovarian cancer via interaction with ATG13 and miR-199a.

Gan X, Zhu H, Jiang X, Obiegbusi SC, Yong M, Long X, Hu J
Molecular cancer 2020 Feb 28;19(1):45

RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia.

Bera R, Chiu MC, Huang YJ, Lin TH, Kuo MC, Shih LY
Journal of hematology & oncology 2019 Oct 22;12(1):104

The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.

Wang D, Diao H, Getzler AJ, Rogal W, Frederick MA, Milner J, Yu B, Crotty S, Goldrath AW, Pipkin ME
Immunity 2018 Apr 17;48(4):659-674.e6

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The RUNX1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human RUNX1. This antibody recognizes human, mouse antigen. The RUNX1 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 25315-1-AP.pdf

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