Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- PB9157 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-RUNX1/AML1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CBFA2/RUNX1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CBFA2/RUNX1 information: Molecular Weight: 48737 MW; Subcellular Localization: Nucleus; Tissue Specificity: Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Brain death induces the alteration of liver protein expression profiles in rabbits.
Du B, Li L, Zhong Z, Fan X, Qiao B, He C, Fu Z, Wang Y, Ye Q
International journal of molecular medicine 2014 Aug;34(2):578-84
International journal of molecular medicine 2014 Aug;34(2):578-84
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of RUNX1 using anti-RUNX1 antibody (PB9157). RUNX1 was detected in paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX1 Antibody (PB9157) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image