701202

antibody from Invitrogen Antibodies

Targeting: RUNX1 AML1, AMLCR1, CBFA2, PEBP2A2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

701202

Provider

Invitrogen Antibodies

Product name

RUNX1 Recombinant Rabbit Monoclonal Antibody (3H2L6)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

3H2L6

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of K562 (Lane 1), PC-3 (Lane 2) and Mouse Small Intestine (Lane 3). The blots were probed with Recombinant Rabbit Monoclonal Anti-RUNX1 Antibody (Product # 701202, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Recombinant Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 60 kDa corresponding to RUNX1 was observed in K562 and PC3, while ~ 48 kDa band was observed in mouse small intestine tissue lysate. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (22834). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RUNX1 in fetal mouse thymus lysate using a RUNX1 recombinant rabbit monoclonal antibody (Product # 701202) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~52kDa.