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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Other assay [2]
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- Product number
- TA347279 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal AML1-ETO Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal AML1-ETO Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RUNX1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using the antibody against AML1-ETO diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against AML1-ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:32,750.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ChIP assays were performed using Kasumi-1 cells, the antibody against AML1-ETO and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. Image shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.
- Validation comment
- Assay
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- a) ChIP-seq results obtained with the ab against AML1-ETO ChIP??s was pooled and analysed with an Illumina Genome Analyzer. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Image shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
- Validation comment
- Assay
