Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB23991 - Provider product page
- Provider
- R&D Systems
- Product name
- Human RUNX1/CBFA2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human RUNX1/CBFA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) RUNX2 or rhRUNX3 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q01196-8
- Isotype
- IgG
- Antibody clone number
- 751926
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references miR-27b attenuates apoptosis induced by transmissible gastroenteritis virus (TGEV) infection via targeting runt-related transcription factor 1 (RUNX1).
Zhao X, Song X, Bai X, Fei N, Huang Y, Zhao Z, Du Q, Zhang H, Zhang L, Tong D
PeerJ 2016;4:e1635
PeerJ 2016;4:e1635
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human RUNX1/CBFA2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human RUNX1/CBFA2 Monoclonal Antibody (Catalog # MAB23991) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RUNX1/CBFA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RUNX1/CBFA2 in K562 Human Cell Line. RUNX1/CBFA2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human RUNX1/CBFA2 Monoclonal Antibody (Catalog # MAB23991) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.