Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-16500 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal FLI1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FLI1 Antibody [NBP2-16500] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FLI1 Antibody [NBP2-16500] - A. 30 ug BCL-1 whole cell lysate/extract 10 % SDS-PAGE FLI1 antibody dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FLI1 Antibody [NBP2-16500] - A. 50 ug rat kidney lysate/extract 10 % SDS-PAGE FLI1 antibody dilution: 1:1000
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: FLI1 Antibody [NBP2-16500] - IP samples: Raji whole cell extract A. 40 ug Raji whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of FLI1 protein by 4 ug FLI1 antibody (NBP2-16500) 10 % SDS-PAGE The immunoprecipitated FLI1 protein was detected by FLI1 antibody (NBP2-16500) diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FLI1 Antibody [NBP2-16500] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.