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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX112937 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112937, RRID:AB_11166318
- Product name
- FLI1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- FLI1 antibody detects FLI1 protein by western blot analysis.A. 50 ?g rat kidney lysate/extract10% SDS-PAGEFLI1 antibody (GTX112937) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FLI1 antibody detects FLI1 protein by western blot analysis.A. 30 ?g BCL-1 whole cell lysate/extract10% SDS-PAGEFLI1 antibody (GTX112937) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FLI1 antibody detects FLI1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 7.5 % SDS-PAGE, and blotted with FLI1 antibody (GTX112937) diluted by 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody (GTX112937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FLI1 antibody detects FLI1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FLI1 protein stained by FLI1 antibody (GTX112937) diluted at 1:500.Blue: Hoechst 33343 staining.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- FLI1 antibody immunoprecipitates FLI1 protein in IP experiments.IP samples: Raji whole cell extractA. 40 £gg Raji whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of FLI1 protein by 4 £gg FLI1 antibody (GTX112937)10 % SDS-PAGEThe immunoprecipitated FLI1 protein was detected by FLI1 antibody (GTX112937) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using FLI1(GTX112937) antibody at 1:500 dilution.
