H00002313-M01

antibody from Abnova Corporation

Targeting: FLI1 EWSR2, SIC-1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002313-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002313-M01, RRID:AB_894080

Product name

FLI1 monoclonal antibody (M01), clone 1G8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant FLI1.

Antigen sequence

MDGTIKEALSVVSDGQSLFDSAYGAAAHLPKADMA
ASGSPDYGQPHKINPLPPQQEWISQPVRVNVKREY
DHMNGSRESPVDCSVSKCSKLVGGGESNPMNYNSY
MDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQ
WLEWAIKEYSLMEIDTSFFQNMDGKELCKMNKEDF
LRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQ
SSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLG
GAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQ
IQLWQFLLELLSDSANASCITWEGTNGEFKMTDPD
EVARRWGERISKPNMNYDKLSRALRYYYDKNIMTK
VHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDI
SYMPSYHAHQQKVNFVPPHPSSMPVTSSSFFGAAS
QYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY


Isotype

IgG

Antibody clone number

1G8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FLI1 expression in transfected 293T cell line by FLI1 monoclonal antibody (M01), clone 1G8.Lane 1: FLI1 transfected lysate(51 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FLI1 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to FLI1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol