H00002313-M05

antibody from Invitrogen Antibodies

Targeting: FLI1 EWSR2, SIC-1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002313-M05

Provider

Invitrogen Antibodies

Product name

FLI1 Monoclonal Antibody (1F6)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1F6

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FLI1 over-expressed 293 cell line, cotransfected with FLI1 Validated Chimera RNAi (Cat # H00002313-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FLI1 monoclonal antibody (M05), clone 1F6 (Cat # H00002313-M05). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of FLI1 expression in transfected 293T cell line by FLI1 monoclonal antibody (M05), clone 1F6.Lane 1: FLI1 transfected lysate(50.982 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to FLI1 on HeLa cell. Antibody concentration is 10 µg/mL.