PA5-29597

antibody from Invitrogen Antibodies

Targeting: FLI1 EWSR2, SIC-1

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-29597 - Provider product page
Provider: Invitrogen Antibodies
Product name: FLI1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: Jurkat, Raji, NCI-H929, BCL-1, rat kidney. Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (83%), Chicken (91%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.22 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

FLI1 protein structure - PA5-29597 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of FLI1 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a FLI1 polyclonal antibody (Product # PA5-29597) diluted at 1:500. Blue: Hoechst 33343 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FLI1 Polyclonal Antibody detects FLI1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FLI1 protein stained by FLI1 Polyclonal Antibody (Product # PA5-29597) diluted at 1:500. Blue: Hoechst 33343 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Friend leukemia integration 1 transcription factor was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FLI1 Polyclonal Antibody (Product # PA5-29597) at (1:100 dilution) in 0.1% BSA, incubated at 4-degree Celsius overnight, and labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents K-562 cells with no expression of FLI1. Panel f represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.