AF6474

antibody from R&D Systems

Targeting: FLI1 EWSR2, SIC-1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6474

Provider

R&D Systems

Product name

Human FLI1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human FLI1 in Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q01543


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Constitutive activation of c-Abl/protein kinase C-δ/Fli1 pathway in dermal fibroblasts derived from patients with localized scleroderma.

Noda S, Asano Y, Akamata K, Aozasa N, Taniguchi T, Takahashi T, Ichimura Y, Toyama T, Sumida H, Yanaba K, Tada Y, Sugaya M, Kadono T, Sato S
The British journal of dermatology 2012 Nov;167(5):1098-105

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human FLI1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 0.5 µg/mL of Human FLI1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FLI1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.