Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-20175 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRMP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human brain tissue lysate.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human brain tissue lysate using a CRMP1 polyclonal antibody (Product # PA5-20175) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CRMP1 in human brain tissue lysate with CRMP1 Polyclonal Antibody (Product # PA5-20175) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CRMP1 Polyclonal Antibody (Product # PA5-20175) and 62, 74 kDa bands corresponding to DRP1 were observed across cell lines and tissues tested except in HEL 92.1.7, Daudi, Mouse and Rat Mammary gland which are reported to be negative. Whole cell extracts (30 µg lysate) of IMR32 (Lane 1), SH-SY5Y (Lane 2), Neuro-2A (Lane 3), HEL 92.1.7 (Lane 4), Daudi (Lane 5), tissue extracts (30 µg lysate) of Mouse Brain (Lane 6), Rat Brain (Lane 7), Mouse Mammary gland (Lane 8) and Rat Mammary gland (Lane 9) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of CRMP1 in Human Brain cells with CRMP1 Polyclonal Antibody (Product # PA5-20175) at 10 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of CRMP1 in Human Brain cells with CRMP1 Polyclonal Antibody (Product # PA5-20175) at 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of CRMP in human brain tissue with CRMP1 Polyclonal Antibody (Product # PA5-20175) at 2 µg/mL.