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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [6]
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- Product number
- PA5-96829 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHRFAM7A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MQKYCIYQHF QFQLLIQHLW IAANCDIADE RFDATFHTNV LVNSSGHCQY LPPGIFKSSC YIDVRWFPFD VQHCKLKFGS WSYGGWSLDL QMQEADISGY IPNGEWDLVG IPGKRSERFY ECCKEPYPDV TFTVTMRRRT LYYGLNLLIP; Positive Samples: A-549, Jurkat, SH-SY5Y, U-87MG, NIH/3T3, Mouse brain, Mouse spinal cord, Rat brain, Rat spinal cord; Cellular Location: Membrane, Multi-pass membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.68 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using CHRFAM7A Polyclonal antibody (Product # PA5-96829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CHRFAM7A in extracts of various cell lines using CHRFAM7A Polyclonal Antibody (Product # PA5-96829) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CHRFAM7A in extracts of various cell lines using CHRFAM7A Polyclonal Antibody (Product # PA5-96829) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHRFAM7A in paraffin-embedded rat brain using CHRFAM7A Polyclonal Antibody (Product # PA5-96829) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHRFAM7A in paraffin-embedded rat kidney using CHRFAM7A Polyclonal Antibody (Product # PA5-96829) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHRFAM7A in paraffin-embedded human prostate using CHRFAM7A Polyclonal Antibody (Product # PA5-96829) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHRFAM7A in paraffin-embedded human stomach using CHRFAM7A Polyclonal Antibody (Product # PA5-96829) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHRFAM7A in paraffin-embedded mouse brain using CHRFAM7A Polyclonal Antibody (Product # PA5-96829) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHRFAM7A in paraffin-embedded mouse stomach using CHRFAM7A Polyclonal Antibody (Product # PA5-96829) at a dilution of 1:200.
