GTX100117

antibody from GeneTex

Targeting: TUBB2A dJ40E16.7, TUBB, TUBB2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX100117

Provider

GeneTex

Proper citation

GeneTex Cat#GTX100117, RRID:AB_10731273

Product name

beta Tubulin 2 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Drosophila

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.A. 30 £gg Jurkat whole cell lysate/extract B. 30 £gg Raji whole cell lysate/extract C. 30 £gg NCI-H929 whole cell lysate/extract 10 % SDS-PAGEbeta Tubulin 2 antibody (GTX100117) dilution: 1:2000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Drosophila lysate 10% SDS PAGE GTX100117 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.A. 30 £gg U87-MG whole cell lysate/extractB. 30 £gg SK-N-SH whole cell lysate/extractC. 30 £gg IMR32 whole cell lysate/extractD. 30 £gg SK-N-AS whole cell lysate/extract10 % SDS-PAGEbeta Tubulin 2 antibody (GTX100117) dilution: 1:2000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.A. 30 £gg C8D30 whole cell lysate/extractB. 30 £gg NIH-3T3 whole cell lysate/extracC. 30 £gg BCL-1 whole cell lysate/extracD. 30 £gg Raw 264.7 whole cell lysate/extractE. 30 £gg C2Cl2 whole cell lysate/extract 10 % SDS-PAGEbeta Tubulin 2 antibody (GTX100117) dilution: 1:2000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.A. 50 £gg rat brain lysate/extract10 % SDS-PAGEbeta Tubulin 2 antibody (GTX100117) dilution: 1:2000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TUBB2A antibody detects TUBB2A protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.Green: TUBB2A protein stained by TUBB2A antibody (GTX100117) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin 2 antibody detects beta Tubulin 2 protein at cytosol on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. beta Tubulin 2 antibody (GTX100117) dilution: 1:500.