H00007280-M02

antibody from Abnova Corporation

Targeting: TUBB2A dJ40E16.7, TUBB, TUBB2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007280-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007280-M02, RRID:AB_10720361

Product name

TUBB2A monoclonal antibody (M02), clone 3C12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant TUBB2A.

Antigen sequence

MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGS
YHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEP
GTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGH
YTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLG
GGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKV
SDTVVEPYNATLSVHQLVENTDETYSIDNEALYDI
CFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQ
LNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQ
YRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAA
IFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
TAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQF
TAMFRRKAFLHWYTGEGMDEMEFTEAESNTNDLVS
EYQQYQDATADEQGEFEEEEGEDEA


Isotype

IgG

Antibody clone number

3C12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

TUBB2A monoclonal antibody (M02), clone 3C12. Western Blot analysis of TUBB2A expression in PC-12(Cat # L012V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged TUBB2A is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human colon. [antibody concentration 6 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol