Antibody data
- Product number
- PA5-27108
- Provider
- Invitrogen Antibodies
- Product name
- Anti-beta Tubulin 2 Polyclonal Antibody
- Provider product page
- Invitrogen Antibodies - PA5-27108
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-27108 targets beta Tubulin 2 in WB applications and shows reactivity with Drosophila and Human samples. The PA5-27108 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 356 of Human beta Tubulin 2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human, Mouse, Rat, Drosophila
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.55 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), PC-12 (Lane 5) RSC96 (Lane 6) tissue extracts of Mouse Lung (Lane 7) and Rat Brain (Lane 8). The blot was probed with Anti-beta Tubulin 2 Rabbit Polyclonal Antibody (Product # PA5-27108, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 52 kDa band corresponding to beta Tubulin 2 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of beta Tubulin 2 showing staining in the cytoskeleton of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min and stained using a beta Tubulin 2 polyclonal antibody (Product # PA5-27108) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of beta Tubulin 2 in the cytosol of paraffin-embedded mouse duodenum tissue using a beta Tubulin 2 polyclonal antibody (Product # PA5-27108) at a dilution of 1:500.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of beta Tubulin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Beta Tubulin 2 Rabbit Polyclonal Antibody (Product # PA5-27108) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive data in Antibodypedia
