PA5-27108

antibody from Invitrogen Antibodies

Targeting: TUBB2A dJ40E16.7, TUBB, TUBB2

Western blot

Antibody data

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Validation data

Product number: PA5-27108 - Provider product page
Provider: Invitrogen Antibodies
Product name: beta Tubulin 2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: Jurkat, Raji, NCI-H929, U87-MG, SK-N-SH, IMR32, SK-N-AS, C8D30, NIH3T3, BCL-1, Raw 264.7, C2Cl2, Drosophila lysate , rat brain. Predicted reactivity: Mouse (100%), Rat (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat, Drosophila
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.55 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TUBB2A protein structure - PA5-27108 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of beta Tubulin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Beta Tubulin 2 Rabbit Polyclonal Antibody (Product # PA5-27108) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of beta Tubulin 2 was performed in HeLa cells fixed in 4% paraformaldehyde/PBS for 15 min. Green: beta Tubulin 2 Polyclonal Antibody (Product # PA5 27108) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of beta Tubulin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Beta Tubulin 2 Rabbit Polyclonal Antibody (Product # PA5-27108) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of beta Tubulin 2 was performed in HeLa cells fixed in 4% paraformaldehyde/PBS for 15 min. Green: beta Tubulin 2 Polyclonal Antibody (Product # PA5 27108) diluted at 1:500. Blue: Hoechst 33342 staining.