- PA5-27108 - Invitrogen Antibodies TUBB2A antibody

Submitted references β(2)-Adrenergic receptor agonist induced hepatic steatosis in mice: modeling nonalcoholic fatty liver disease in hyperadrenergic states.

Shi Y, Pizzini J, Wang H, Das F, Abdul Azees PA, Ghosh Choudhury G, Barnes JL, Zang M, Weintraub ST, Yeh CK, Katz MS, Kamat A
American journal of physiology. Endocrinology and metabolism 2021 Jul 1;321(1):E90-E104

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of beta Tubulin 2 was performed by separating 30 µg of various whole cell lysates/extracts by 10 % SDS-PAGE. Proteins were transferred to a membrane and probed with a beta Tubulin 2 Polyclonal Antibody (Product # PA5-27108) at a dilution of 1:2000. A. U87-MG, B. SK-N-SH, C. IMR32, D. SK-N-AS.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), PC-12 (Lane 5) RSC96 (Lane 6) tissue extracts of Mouse Lung (Lane 7) and Rat Brain (Lane 8). The blot was probed with Anti-beta Tubulin 2 Rabbit Polyclonal Antibody (Product # PA5-27108, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 52 kDa band corresponding to beta Tubulin 2 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of beta Tubulin 2 was performed by separating 30 µg of various whole cell lysates/extracts by 10 % SDS-PAGE. Proteins were transferred to a membrane and probed with a beta Tubulin 2 Polyclonal Antibody (Product # PA5-27108) at a dilution of 1:2000. A. C8D30, B. NIH-3T3, C. BCL-1, D. Raw 264.7, E. C2Cl2 .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of beta Tubulin 2 was performed by separating 30 µg of various whole cell lysates/extracts by 10 % SDS-PAGE. Proteins were transferred to a membrane and probed with a beta Tubulin 2 Polyclonal Antibody (Product # PA5-27108) at a dilution of 1:2000. A. Jurkat, B. Raji , C. NCI-H929 .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of beta Tubulin 2 was performed by separating 30 µg of various whole cell lysates/extracts by 10 % SDS-PAGE. Proteins were transferred to a membrane and probed with a beta Tubulin 2 Polyclonal Antibody (Product # PA5-27108) at a dilution of 1:2000. A. U87-MG, B. SK-N-SH, C. IMR32, D. SK-N-AS.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of beta Tubulin 2 was performed by separating 30 µg of Drosophila lysate lysates by 10% SDS PAGE. Proteins were transferred to a membrane and probed with a beta Tubulin 2 Polyclonal Antibody (Product # PA5-27108) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of beta Tubulin 2 was performed by separating 50 µg of rat brain lysates/extracts by 10 % SDS-PAGE. Proteins were transferred to a membrane and probed with a beta Tubulin 2 Polyclonal Antibody (Product # PA5-27108) at a dilution of 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of beta Tubulin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Beta Tubulin 2 Rabbit Polyclonal Antibody (Product # PA5-27108) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of beta Tubulin 2 was performed in HeLa cells fixed in 4% paraformaldehyde/PBS for 15 min. Green: beta Tubulin 2 Polyclonal Antibody (Product # PA5 27108) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: beta Tubulin 2 Polyclonal Antibody detects beta Tubulin 2 protein at cytosol on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Beta Tubulin 2 Polyclonal Antibody (Product # PA5-27108) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-27108

Antibody data