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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- OAAB01422 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- VIM antibody - center region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of Vimentin (arrow) using Vimentin Antibody (Center) either nontransfected (Lane 1) or lysates transiently transfected transfected with the VIM gene (Lane 2) (Origene Technologies).
- Sample type
- lysatestransiently transfected with the VIM gene
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of Vimentin Antibody (Center) in A2058,A375 cell lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab.
- Sample type
- A2058,A375 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Sample type
- human breast carcinoma tissue
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
