- UM800055 - OriGene VIM antibody

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Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Confocal immunofluoresce image of primary rat neurons labeled with anti-Vimentin mouse monoclonal antibody (UM800055, green, 1:100) with DAPI (blue) for nuclear. Scale bar, 10um.
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunofluorescent staining of HeLa cells using anti-Vimentin mouse monoclonal antibody (UM800055, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human tonsil using anti-VIM mouse monoclonal antibody. (UM800055; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human glioma using anti-vimentin clone UMAB160 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800055 requires HIER with with Accel 3in1 EDTA solution pH8.7 at 110C for 3 min using pressure chamber/cooker. The neural cells shows membrane and cytoplasmic staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human tonsil using anti-Vimentin clone UMAB160 mouse monoclonal antibody (UM800055) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer usin
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human three cases of gastric cancer using anti-Vimentin clone UMAB160 mouse monoclonal antibody (UM800055) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human skin using anti-Vimentin clone UMAB160 mouse monoclonal antibody (UM800055) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human placenta using anti-Vimentin clone UMAB160 mouse monoclonal antibody (UM800055) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer us
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: IHC staining of three cases of paraffin-embedded human ovarain cancer using anti-Vimentin clone UMAB160 mouse mAb (UM800055) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressur
Validation comment: IHC

UM800055

Antibody data