Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- R31772 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Vimentin Antibody
- Antibody type
- Polyclonal
- Antigen
- An amino acid sequence from the C-terminus of human Vimentin (DTHSKRTLLIKTVETRDGQVINETSQHHDDLE) was used as the immunogen for this Vimentin antibody (100% homologous in human, mouse and rat).
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the Vimentin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Vimentin antibody and Lane 1: HT1080; 2: NIH3T3; 3: Jurkat; 4: HUT; 5: MCF-7; 6: HeLa; 7: human placenta; 8: rat testis; 9: mouse testis lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Vimentin antibody testing of human breast cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Vimentin antibody testing of mouse heart
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Vimentin antibody testing of rat heart
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Vimentin antibody testing of mouse kidney. Required HIER: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Vimentin antibody testing of rat kidney. Required HIER: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 min.