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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- F48163 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- VIM Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 152-181 from the human protein was used as the immunogen for this VIM antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- For long term storage, aliquot the VIM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of VIM antibody and A2058, A375 lysate.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of VIM antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human breast carcinoma tissue stained with VIM antibody
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- VIM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- VIM antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC secondary Ab was used.
