Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX27752 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX27752, RRID:AB_373289
- Product name
- Vimentin antibody [VI-01]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster, Porcine
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody GTX27752. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)
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- GeneTex (provider)
- Main image
- Experimental details
- Western Blot analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4). Lane 1,2: immunostaining with anti-Vimentin (GTX27752) Lane 3,4: immunostaining with anti-human Vimentin (GTX23974)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (GTX27752): Dyomics 547. Nuclei are stained with DAPI (blue)
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (GTX27752): Dyomics 547. Nuclei are stained with DAPI (blue)