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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- ARG65724 - Provider product page
- Provider
- Arigo
- Product name
- anti-Vimentin antibody
- Antibody type
- Monoclonal
- Antigen
- KLH-conjugated synthetic peptide of Human Vimentin (NP_003371.2).
- Description
- Purification with Protein A.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- B4-B11
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 1) A549, 2) HeLa, 3) NIH/3T3, and 4) Mouse embryonic stem cell lysates stained with ARG65724 anti-Vimentin antibody at 1:2000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: HeLa cells stained with ARG65724 anti-Vimentin antibody (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Human lung tissue stained with ARG65724 anti-Vimentin antibody. Counter stained with hematoxylin.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Human colon carcinoma tissue stained with ARG65724 anti-Vimentin antibody.Counter stained with hematoxylin.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Human stomach carcinoma tissue stained with ARG65724 anti-Vimentin antibody. Counter stained with hematoxylin.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Mouse lung tissue stained with ARG65724 anti-Vimentin antibody. Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG65724 anti-Vimentin antibody at 1:50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
