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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- F54035 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Vimentin Antibody
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 4F8-A5-H5
- Vial size
- 0.1 ml
- Storage
- Store the Vimentin antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Vimentin-positive cell lines (Molt-4, K562, COS7, Jurkat, HeLa) and Vimentin-negative cell lines (Daudi, Ramos, Raji) using Vimentin antibody at 1:1000. Predicted molecular weight ~58 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF staining of HeLa cells, fixed with 4% Paraformaldehyde, using Vimentin antibody at 1:800.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation of Vimentin from HeLa cell lysate, and subsequent western blot testing, using Vimentin antibody.
