Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP2-52867 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Vimentin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human Vimentin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa] Lane 2: Human tonsil tissue lysate
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Vimentin Antibody (CL0157) [NBP2-52867] - Analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human kidney shows strong immunoreactivity in renal glomeruli but not tubuli cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human prostate shows strong immunoreactivity in the connective tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human cerebellum shows immunoreactivity in the endothelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Vimentin Antibody (CL0157) [NBP2-52867] - Staining of human skin shows distinct positivity in fibroblasts.