Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [6]
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- Product number
- 42-9897-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Vimentin Monoclonal Antibody (V9), eFluor™ 615, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, as well as pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils. This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin. Applications Reported: This V9 antibody has been reported for use in immunocytochemistry and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P). Applications Tested: This V9 antibody has been tested by immunocytochemistry on fixed and permeabilized C6 cells at less than or equal to 1 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Rat, Canine, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- V9
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Characterization of epithelial cells, connective tissue cells and immune cells in human upper airway mucosa by immunofluorescence multichannel image cytometry: a pilot study.
MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis.
Omentin-1 is associated with atrial fibrillation in patients with cardiac valve disease.
GMP-compatible and xeno-free cultivation of mesenchymal progenitors derived from human-induced pluripotent stem cells.
Levels of hepatic Th17 cells and regulatory T cells upregulated by hepatic stellate cells in advanced HBV-related liver fibrosis.
Giotakis AI, Dudas J, Glueckert R, Dejaco D, Ingruber J, Fleischer F, Innerhofer V, Pinggera L, Bektic-Tadic L, Gabriel SAM, Riechelmann H
Histochemistry and cell biology 2021 Mar;155(3):405-421
Histochemistry and cell biology 2021 Mar;155(3):405-421
MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis.
Zeng X, Liao H, Wang F
Cytotechnology 2021 Apr;73(2):203-215
Cytotechnology 2021 Apr;73(2):203-215
Omentin-1 is associated with atrial fibrillation in patients with cardiac valve disease.
Chen Y, Liu F, Han F, Lv L, Tang CE, Xie Z, Luo F
BMC cardiovascular disorders 2020 May 6;20(1):214
BMC cardiovascular disorders 2020 May 6;20(1):214
GMP-compatible and xeno-free cultivation of mesenchymal progenitors derived from human-induced pluripotent stem cells.
McGrath M, Tam E, Sladkova M, AlManaie A, Zimmer M, de Peppo GM
Stem cell research & therapy 2019 Jan 11;10(1):11
Stem cell research & therapy 2019 Jan 11;10(1):11
Levels of hepatic Th17 cells and regulatory T cells upregulated by hepatic stellate cells in advanced HBV-related liver fibrosis.
Li X, Su Y, Hua X, Xie C, Liu J, Huang Y, Zhou L, Zhang M, Li X, Gao Z
Journal of translational medicine 2017 Apr 11;15(1):75
Journal of translational medicine 2017 Apr 11;15(1):75
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry on fixed and permeabilized C6 cells using 1 µg/mL Mouse IgG1 K Isotype Control eFluor® 615 (left) or 1 µg/mL Anti-Vimentin eFluor® 615 (right). Nuclei are counterstained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Supernatants from HSC increased the percentages of Th17 cells and Tregs. a Phenotypes of primary HSC extracted from HBV-related fibrotic liver tissues in Group 2. Sections were immunostained with desmin, FAP, FSP, vimentin, fibronectin and a-SMA antibodies. One of 20 representative micrographs is shown. b , d Purified CD4+ T cells were cultured alone (Blank) or with 30% indicated supernatants. The values in the quadrants represent the percentages of Th17 cells and Tregs. The data shown are representative dot plots from more than three independent experiments. c , e The statistical analysis of the effect of LX-2 and pHSC supernatant on the percentages of Th17 cells ( c ) and Tregs ( e ). * p < 0.05, ** p < 0.01, *** p < 0.001
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 miR-384 mimic inhibits EMT, migration and invasion of NPC cells. a levels of mesenchyme marker protein Vimentin and epithelium-marker protein E-cadherin in cells measured by western blot analysis; b , c number of migrated ( b ) and invaded ( c ) cells determined by Transwell assays. Data are exhibited as mean +- SD from three independent experiments; in panel ( a ), data were analyzed using two-way ANOVA, while data in panels ( b ) and ( c ) were analyzed by one-way ANOVA, and Tukey''s multiple comparison test was used for the post-hoc test after ANOVA; * p < 0.05; ** p < 0.01 vs. the Mock group; # p < 0.05 vs. the InC group
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Omentin-1 inhibited TGF-beta1-induced endothelial-mesenchymal transition (EndMT) of human umbilical vein endothelial cells (HUVECs). Vimentin and VE-Cad protein levels in HUVECs were detected via western blotting ( a ). The mRNA levels of vimentin and VE-Cad were detected via RT-qPCR ( b ). Expression of mRNA were normalized to GAPDH. Representative images of immunofluorescence staining for vimentin ( c ) and VE-Cad ( d ) (200x magnification) in HUVECs. The p-SMAD3 and t-SMAD3 protein levels in HUVECs were detected via western blotting ( e ). ( f ) Representative images of HUVECs scratch assay (x 100 magnification). The values were mean +- SEM of three independent experiments. * P < 0.05 vs control group, ** P < 0.01 vs control group, *** P < 0.001 vs control group, + P < 0.05 vs TGF-beta1 group, ++ P < 0.01 vs TGF-beta1 group, +++ P < 0.001 vs TGF-beta1 group
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Chronic rhinosinusitis types and normal tissue; comparison of bright-field and fluorescent-immunolabeled sections. H.E. staining (a, c, e) and immunofluorescence staining (b, d, f) of control (a, b) , chronic rhinosinusitis without polyps (CRSsNP) (c, d) and chronic rhinosinusitis with polyps (CRSwNP) (e, f) tissue. Four-channel fluorescence represents nuclear DAPI staining (blue), cytokeratin (green), vimentin (red) and CD45 combined with CD18 (yellow). Scale bar: 100 mum
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Comparison of histograms of fluorescence signals between isotype controls and patient's tissue. Histograms of fluorescence signals after control incubation in the upper histograms (isotype controls) and specific fluorescence signals after test incubation in the lower histograms (patient's tissue). Presentation of a random cell sample of all patients and their isotype controls. X -axis: mean intensity in logarithmic scale. Y -axis: cell count. The left histograms represented cytokeratin fluorescence signal, the middle histograms vimentin fluorescence signal and the right histograms CD45/CD18 fluorescence signal
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Cytoskeletal proteins. Cell painting showing the production of the cytoskeletal proteins F-actin and vimentin in induced pluripotent stem cell-derived mesodermal progenitors (line 1013A and BC1) cultured in different media at passages 6, 10, and 15. The nuclei are stained with 4',6-diamidino-2-phenylindole (blue). Scale bar = 20 mum. Abbreviations: MP, mesodermal progenitors; FBS, fetal bovine serum; HPL, human platelet lysate; P, passage. Additional data are shown in Additional file 4 : Figure S3