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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- LS-C747196 - Provider product page
- Provider
- LSBio
- Product name
- Vimentin Antibody LS-C747196
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using VIM antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of extracts from normal (control) and VIM knockout (KO) 293T cells, using VIM antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsil using VIM antibody at dilution of 1:150 (40x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsil using VIM antibody at dilution of 1:150 (40x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach using VIM antibody at dilution of 1:150 (40x lens).
