Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Protein array [1]
- Other assay [1]
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Validation data
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- Product number
- V3935 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Vimentin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human, Bovine, Canine, Feline, Goat, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- VM452
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- Store the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Vimentin antibody and Raji cell lysate. Expected molecular weight: 53-58 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human melanoma with Vimentin antibody (clone VM452). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of Jurkat cells and Alexa Fluor 488 conjugated Vimentin antibody (green), cells alone (black) and isotype control (gray).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Vimentin antibody (clone VM452). These results demonstrate the foremost specificity of the VM452 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free Vimentin antibody (clone VM452) as confirmation of integrity and purity.