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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Western blot [1]
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- Product number
- M00235-4 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Vimentin Antibody
- Antibody type
- Polyclonal
- Description
- Anti-Vimentin Antibody tested in WB, ICC, IF, IHC, reactive to Human, rat, mouse, cow, pig, horse
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Rabbit
- Vial size
- 100ul
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- Store at 4℃ for short term, for longer term at -20℃. Avoid freeze / thaw cycles.
Submitted references AKT/GSK-3beta/VEGF signaling is involved in P2RY2 activation-induced the proliferation and metastasis of gastric cancer.
A microfluidic lung-on-a-chip based on biomimetic hydrogel membrane.
Effect of TGF-β2 on the Mechanical Properties of Posterior Scleral Fibroblasts in Experimental Myopia.
Periodic Heat Stress Licenses EMSC Differentiation into Osteoblasts via YAP Signaling Pathway Activation.
Effects of curcumin nanoparticles on proliferation and VEGF expression of human retinal pigment epithelial cells.
Gentiopicroside Ameliorates Diabetic Renal Tubulointerstitial Fibrosis via Inhibiting the AT1R/CK2/NF-κB Pathway.
Gentianella acuta improves TAC-induced cardiac remodelling by regulating the Notch and PI3K/Akt/FOXO1/3 pathways.
Hepatitis B virus X mediates podocyte pyroptosis by regulating the ROS/NLRP3 signaling pathway in hepatitis B virus-associated glomerulonephritis.
Extracellular vesicles delivering nuclear factor I/C for hard tissue engineering: Treatment of apical periodontitis and dentin regeneration.
A novel approach to the identification and enrichment of cancer stem cells from a cultured human glioma cell line.
Human umbilical cord blood-derived non-hematopoietic stem cells suppress lymphocyte proliferation and CD4, CD8 expression.
Dong CR, Hu DX, Liu SC, Luo HL, Zhang WJ
Carcinogenesis 2023 May 15;44(1):65-79
Carcinogenesis 2023 May 15;44(1):65-79
A microfluidic lung-on-a-chip based on biomimetic hydrogel membrane.
Shen C, Yang H, She W, Meng Q
Biotechnology and bioengineering 2023 Jul;120(7):2027-2038
Biotechnology and bioengineering 2023 Jul;120(7):2027-2038
Effect of TGF-β2 on the Mechanical Properties of Posterior Scleral Fibroblasts in Experimental Myopia.
Chen B, Chen W, Han B, Tong C, Ma J
BioMed research international 2022;2022:6193876
BioMed research international 2022;2022:6193876
Periodic Heat Stress Licenses EMSC Differentiation into Osteoblasts via YAP Signaling Pathway Activation.
Shi W, Wang Z, Bian L, Wu Y, HuiYa M, Zhou Y, Zhang Z, Wang Q, Zhao P, Lu X
Stem cells international 2022;2022:3715471
Stem cells international 2022;2022:3715471
Effects of curcumin nanoparticles on proliferation and VEGF expression of human retinal pigment epithelial cells.
Zheng HS, Lan YQ, Zhong XW, Zhou HS, Xu JY
International journal of ophthalmology 2022;15(6):905-913
International journal of ophthalmology 2022;15(6):905-913
Gentiopicroside Ameliorates Diabetic Renal Tubulointerstitial Fibrosis via Inhibiting the AT1R/CK2/NF-κB Pathway.
Xu Z, Zhang M, Wang Y, Chen R, Xu S, Sun X, Yang Y, Lin Z, Wang S, Huang H
Frontiers in pharmacology 2022;13:848915
Frontiers in pharmacology 2022;13:848915
Gentianella acuta improves TAC-induced cardiac remodelling by regulating the Notch and PI3K/Akt/FOXO1/3 pathways.
Zhou WW, Dai C, Liu WZ, Zhang C, Zhang Y, Yang GS, Guo QH, Li S, Yang HX, Li AY
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022 Oct;154:113564
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022 Oct;154:113564
Hepatitis B virus X mediates podocyte pyroptosis by regulating the ROS/NLRP3 signaling pathway in hepatitis B virus-associated glomerulonephritis.
Yu Y, Dong H, Sun J, Li B, Chen Y, Feng M, Yang X, Jiang W
Iranian journal of basic medical sciences 2022 Jan;25(1):103-109
Iranian journal of basic medical sciences 2022 Jan;25(1):103-109
Extracellular vesicles delivering nuclear factor I/C for hard tissue engineering: Treatment of apical periodontitis and dentin regeneration.
Yang S, Liu Q, Chen S, Zhang F, Li Y, Fan W, Mai L, He H, Huang F
Journal of tissue engineering 2022 Jan-Dec;13:20417314221084095
Journal of tissue engineering 2022 Jan-Dec;13:20417314221084095
A novel approach to the identification and enrichment of cancer stem cells from a cultured human glioma cell line.
Zhou ZH, Ping YF, Yu SC, Yi L, Yao XH, Chen JH, Cui YH, Bian XW
Cancer letters 2009 Aug 18;281(1):92-9
Cancer letters 2009 Aug 18;281(1):92-9
Human umbilical cord blood-derived non-hematopoietic stem cells suppress lymphocyte proliferation and CD4, CD8 expression.
Ji F, Wang Y, Sun H, Du J, Zhao H, Wang D, Xu Q, Duan D, Yang H
Journal of neuroimmunology 2008 Jul 15;197(2):99-109
Journal of neuroimmunology 2008 Jul 15;197(2):99-109
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of whole cell lysates using rabbit pAb to vimentin, M00235-4, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa.
- Additional image
