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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- Ab156188 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Vimentin Mouse mAb
- Antibody type
- Monoclonal
- Description
- ApplicationWB: 1/2,000-1/20,000 ; Predicted molecular weight: 54 kDaFlow: 0.016 _g/mL Ð 2 _g/mLICC/IF: 2 _g/mL
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- AB10/4F11
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C for short term (1-2 weeks). Store at -20¡C or -80¡C. Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Vimentin Mouse mAb (Ab156188) - Western Blot All lanes: Vimentin Antibody (Ab156188) at 0.5 μg/mL Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 53.7 kDa Observed band size: 54.9 kDa Exposure time: 26.9 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Vimentin Mouse mAb (Ab156188) - ICC/IF IF analysis of Vimentin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vimentin Mouse mAb (Ab156188) at 2 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Vimentin Mouse mAb (Ab156188) - Flow Cytometry Overlay histogram showing HeLa cells stained with Vimentin Mouse mAb (Ab156188) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control.
