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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- Ab179919 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Vimentin Mouse mAb
- Antibody type
- Monoclonal
- Description
- ApplicationWB: 1/2,000-1/100,000 ; Predicted molecular weight: 54 kDaFlow: 0.016 _g/mL - 0.5 _g/mL
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- AB08/4A9
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4¡C for short term (1-2 weeks). Store at -20¡C or -80¡C. Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Vimentin Mouse mAb (Ab179919) - Western Blot All lanes: Vimentin Antibody (Ab179919) at 0.5 μg/mL Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 53.7 kDa Observed band size: 54.9 kDa Exposure time: 20.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Vimentin Mouse mAb (Ab179919) - Flow Cytometry Overlay histogram showing HeLa cells stained with Vimentin Mouse mAb (Ab179919) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control.
