NBP1-85814

antibody from Novus Biologicals

Targeting: VIM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85814

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85814, RRID:AB_11020836

Product name

Rabbit Polyclonal Vimentin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Vimentin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Changes in cannabinoid receptors, aquaporin 4 and vimentin expression after traumatic brain injury in adolescent male mice. Association with edema and neurological deficit.

Lopez-Rodriguez AB, Acaz-Fonseca E, Viveros MP, Garcia-Segura LM
PloS one 2015;10(6):e0128782

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Tumor suppressive microRNA‑138 contributes to cell migration and invasion through its targeting of vimentin in renal cell carcinoma.

Yamasaki T, Seki N, Yamada Y, Yoshino H, Hidaka H, Chiyomaru T, Nohata N, Kinoshita T, Nakagawa M, Enokida H
International journal of oncology 2012 Sep;41(3):805-17

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: Vimentin Antibody [NBP1-85814] - Electropherogram image(s) of corresponding Simple Western lane view. Vimentin antibody was used at 1:20 dilution on NIH-3T3 lysates(s).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Vimentin Antibody [NBP1-85814] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Vimentin Antibody [NBP1-85814] - Analysis in human cell lines U-251MG and MCF-7 using anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human skeletal muscle shows no positivity in myocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human ovary shows moderate cytoplasmic positivity in stromal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Analysis in human ovary and skeletal muscle tissues. Corresponding VIM RNA-seq data are presented for the same tissues.