Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Other assay [3]
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Validation data
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- Product number
- MA1-10459 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Vimentin Monoclonal Antibody (VI-10)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types. Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Porcine
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- VI-10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Expansion of Circulating Tumor Cells from Patients with Locally Advanced Pancreatic Cancer Enable Patient Derived Xenografts and Functional Studies for Personalized Medicine.
Heterogeneity of Human Breast Stem and Progenitor Cells as Revealed by Transcriptional Profiling.
Quantitative detection of human spermatogonia for optimization of spermatogonial stem cell culture.
Rivera-Báez L, Lohse I, Lin E, Raghavan S, Owen S, Harouaka R, Herman K, Mehta G, Lawrence TS, Morgan MA, Cuneo KC, Nagrath S
Cancers 2020 Apr 20;12(4)
Cancers 2020 Apr 20;12(4)
Heterogeneity of Human Breast Stem and Progenitor Cells as Revealed by Transcriptional Profiling.
Colacino JA, Azizi E, Brooks MD, Harouaka R, Fouladdel S, McDermott SP, Lee M, Hill D, Madden J, Boerner J, Cote ML, Sartor MA, Rozek LS, Wicha MS
Stem cell reports 2018 May 8;10(5):1596-1609
Stem cell reports 2018 May 8;10(5):1596-1609
Quantitative detection of human spermatogonia for optimization of spermatogonial stem cell culture.
Zheng Y, Thomas A, Schmidt CM, Dann CT
Human reproduction (Oxford, England) 2014 Nov;29(11):2497-511
Human reproduction (Oxford, England) 2014 Nov;29(11):2497-511
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), Hep G2 (Lane 2), SHSY-5Y (Lane 3), A-431 (Lane 4) and HeLa (Lane 5). The blot was probed with Anti-Vimentin Monoclonal Antibody (Product # MA1-10459, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 54 kDa band corresponding to Vimentin was observed across all the cell lines positive for Vimentin (Lanes 1, 3 and 5) while this band was absent in the cell lines which do not express Vimentin protein (Lanes 2 and 4).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human vimentin using mouse monoclonal antibody VI-10 on lysates of MOLT-4 cell line (low expression), U87 cell line (positive) and Raji cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-vimentin Monoclonal antibody (Product # MA1-10459) followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for vimentin at approximately 55kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin (TU-01), vimentin (green) with anti-Vimentin (VI-10) Monoclonal antibody (Product # MA1-10459). Nuclei are stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Vimentin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vimentin Mouse Monoclonal Antibody (Product # MA1-10459) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L)/IgM (L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic, cytoskeletal and nuclear localization. Panel e represents negative control, A-431 cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human skin (paraffin sections) using anti-vimentin (VI-10) Monoclonal antibody (Product # MA1-10459).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Unbiased Analysis of Single-Cell Gene Expression Data from ALDH + Breast Cells (A) Hierarchical clustering analysis of gene expression measures from a total of 105 ALDH + cells isolated from three independent individuals reveals four expression clusters. (B) Violin plot analysis of the expression of the gene expression panel across the four clusters. (C) Comparison of expression of the epithelial gene CDH1 (red) and the mesenchymal gene VIM (blue) across the four clusters. (D) Immunofluorescence antibody staining of adjacent normal breast tissue for DAPI, ALDH1A1, ALDH1A3, CK8/18, and vimentin. Arrow identifies cell with co-expression of CK8/18, vimentin, and ALDH1A3. Scale bars, 10 mum.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by VI-01 Monoclonal antibody (Product # MA1-10459). IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions); Lnr = lysate (non-reducing conditions); IPr = immunoprecipitate (reducing conditions); IPnr = immunoprecipitate (non-reducing conditions).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Characterizing CTC-derived cell lines. ( A ) Brightfield images (10x and 40x) of expanded CTC cultures. ( B ) Percentage of epithelial-to-mesenchymal transition (EMT)-like CTC in adherent cultures. ( C ) Growth curve analysis of adhered CTC cultures (n = 3). ( D ) Representative images of immunofluorescence staining using cytokeratin (red)/vimentin (magenta)/CD44 (light blue)/CD45 (green)/DAPI (blue). (Scale bar = 50 um).